Shortage of donor organs for transplantation has led the field of Regenerative Medicine to explore the possibility to produce organs in vitro from a source of embryonic stem cells. A few labs have shown that they can sometimes produce parts of organs or miniature organs by soaking stem cells in various culture media. We use a different and innovative approach, based on our past results and expertise allowing us to take control of embryonic stem cells, imposing them a precise set of information. The goal of this project is to define optimal conditions to induce mouse embryonic stem cells to start differentiating and organizing themselves 1) into full tissues, and 2) in a reproducible manner. This research program offers a very promising path towards differentiation of cells into tissues and organs in vitro, bringing a wealth of information to the Stem Cell Biology and Regenerative Medicine fields, moving closer to the goal of producing functional organs in vitro for medical purposes.